SR-BI/SR-BII Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB400-134

Novus Biologicals, part of Bio-Techne
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NB400-134
NB400-134SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen (Negative), Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

BN, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all SR-BI/SR-BII Antibodies »

Product Specifications

Immunogen

A peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII that was expressed as two tandem copies in bacteria using the pET system.

Reactivity Notes

Rat (PMID: 22097902) and Human (PMID: 17105723) reactivity reported ins scientific literature

Localization

Lysosome; lysosomal membrane; multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SR-BI/SR-BII Antibody

Western Blot: SR-BI/SR-BII Antibody [NB400-134]

Western Blot: SR-BI/SR-BII Antibody [NB400-134]

Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.
Immunocytochemistry/ Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134]

Immunocytochemistry/ Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134]

Immunocytochemistry/Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134]

Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134]

Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using 5 ug/ml concentration of SR-BI/SR-BII antibody. Specific and expected granular membrane-cytoplasmic staining was observed in the hepatocytes. [40X Magnification]
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Applications for SR-BI/SR-BII Antibody

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 24859737)

Flow Cytometry

1:400. Use reported in scientific literature (PMID 22097902)

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen (Negative)

5 ug/ml

Western Blot

1:1000
Application Notes
In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI and/or SR-BII such as liver, ovary, adrenal glands, and to as lesser extent testes, heart and mammary glands.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB400-134 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SR-BI/SR-BII

SR-BI (scavenger receptor class BI, SCARB1) is a member of the CD36 receptor family and acts as a major receptor for HDL for facilitating selective lipid uptake from lipoproteins in several tissues. The SR-BI gene gives rise to at least two mRNA splice variants (SR-BI and SR-BII), with the SR-BII variant being the main isoform in terms of mRNA levels in certain tissues, for example SR-BII mRNA is 48-fold higher in concentration than SR-BI in brain cells. Similar differential SR-BII protein expression levels have also been detected in considerable amounts in other tissues, such as testes, retinal pigment epithelial cells, and spleen. SR-BII isoform differs from SR-BI only in its entirely different, yet highly conserved, cytoplasmic C-terminus which has been proposed to contains a signal responsible for marked alterations in cellular distribution as well as cellular trafficking of SR-BII compared with SR-BI. In contrast to SR-BI, SR-BII mediates the rapid uptake of significant amounts of HDL into endosomal recycling compartment by a pathway which is different from selective lipid uptake at the cell surface (mediated by SR-BI). Because SR-BII influences cellular cholesterol trafficking/homeostasis in a manner distinct from SR-BI, the relative expression as well as functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Alternate Names

CD36 and LIMPII analogous 1, CD36 antigen, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1, CD36L1scavenger receptor class B type 1, CLA1CD36 antigen-like 1, CLA-1HDLQTL6, Collagen type I receptor, thrombospondin receptor-like 1, scavenger receptor class B type III, scavenger receptor class B, member 1, SRB1scavenger receptor class B member 1, SR-BIMGC138242

Entrez Gene IDs

20778 (Mouse)

Gene Symbol

SCARB1

UniProt

O35114, Q61009 (Mouse)

Additional SR-BI/SR-BII Products

Product Documents for SR-BI/SR-BII Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SR-BI/SR-BII Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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