Src Antibody (5A18) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19188
![Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]](https://resources.bio-techne.com/images/products/Src-Antibody-5A18-Immunohistochemistry-NBP1-19188-img0001.jpg "Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]")
![Western Blot: Src Antibody (5A18) [NBP1-19188]](https://resources.bio-techne.com/images/products/Src-Antibody-5A18-Western-Blot-NBP1-19188-img0004.jpg "Western Blot: Src Antibody (5A18) [NBP1-19188]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse Clone # 5A18
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human full length c-Src
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
Src (5A18)
Clonality
Monoclonal
Host
Mouse
Scientific Data Images for Src Antibody (5A18) - BSA Free
![Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]](https://resources.bio-techne.com/images/products/Src-Antibody-5A18-Immunohistochemistry-NBP1-19188-img0002.jpg "Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]")
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human prostate tissue stained with Src, mAb (5A18) at 20 ug/ml.![Western Blot: Src Antibody (5A18) [NBP1-19188]](https://resources.bio-techne.com/images/products/Src-Antibody-5A18-Western-Blot-NBP1-19188-img0003.jpg "Western Blot: Src Antibody (5A18) [NBP1-19188]")
Western Blot: Src Antibody (5A18) [NBP1-19188]
Western Blot: Src Antibody (5A18) [NBP1-19188] - Western blot analysis of Src using 20 ug of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1 ug/ml.Applications for Src Antibody (5A18) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1 ug/ml
Application Notes
WB validation from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: tested in A431 -/+ EGF; antibody dilution of 1:50; separated by size
See Simple Western Antibody Database for Simple Western validation: tested in A431 -/+ EGF; antibody dilution of 1:50; separated by size
Reviewed Applications
Read 2 reviews rated 5 using NBP1-19188 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.2) and 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Src
Long Name
v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog
Alternate Names
ASV, c-Src, RSVgp4
Gene Symbol
SRC
UniProt
Additional Src Products
Product Documents for Src Antibody (5A18) - BSA Free
Product Specific Notices for Src Antibody (5A18) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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