Src [p Tyr530] Antibody (S03-8H8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20066

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20066-100ul
NBP3-20066-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-8H8

Concentration

0.3 mg/ml

View all Src Antibodies »

Product Specifications

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src (Uniprot # P12931)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Src [p Tyr530] Antibody (S03-8H8)

Western Blot: Src [p Tyr530] Antibody (S03-8H8) [NBP3-20066] - Western blot detection of Src (phospho-Tyr529) in CHO-K1 cell lysates using NBP3-20066 (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.

Western Blot: Src [p Tyr530] Antibody (S03-8H8) [NBP3-20066] - Western blot detection of Src (phospho-Tyr529) in CHO-K1 cell lysates using NBP3-20066 (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.

Applications for Src [p Tyr530] Antibody (S03-8H8)

Application
Recommended Usage

Western Blot

1/2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Src

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Alternate Names

ASV, c-Src, RSVgp4

Gene Symbol

SRC

Additional Src Products

Product Documents for Src [p Tyr530] Antibody (S03-8H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Src [p Tyr530] Antibody (S03-8H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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