SRD5A2 Antibody (8A4H5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15900
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8A4H5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRD5A2 (P31213). MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SRD5A2 Antibody (8A4H5)
![Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900]](https://resources.bio-techne.com/images/products/SRD5A2-Antibody-8A4H5-Western-Blot-NBP3-15900-img0003.jpg "Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900]")
Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900]
Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Analysis of extracts of various cell lines, using SRD5A2 Rabbit mAb (NBP3-15900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] -
Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Analysis of paraffin-embedded mouse testis using SRD5A2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] -
Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Analysis of paraffin-embedded rat testis using SRD5A2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for SRD5A2 Antibody (8A4H5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:100 - 1:400
Immunohistochemistry-Paraffin
1:500
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SRD5A2
Long Name
3-oxo-5-alpha-steroid 4-dehydrogenase 2
Alternate Names
5 alpha-SR2, S5AR 2, SR type 2
Gene Symbol
SRD5A2
Additional SRD5A2 Products
Product Documents for SRD5A2 Antibody (8A4H5)
Product Specific Notices for SRD5A2 Antibody (8A4H5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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