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SRD5A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-981

Novus Biologicals, part of Bio-Techne
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NB300-981

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Hamster IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence QVQCQQSPVLAG corresponding to N-Terminus according to NP_000339.2.

Clonality

Polyclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for SRD5A2 Antibody

Western Blot: SRD5A2 Antibody [NB300-981]

Western Blot: SRD5A2 Antibody [NB300-981]

Western Blot: SRD5A2 Antibody [NB300-981] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SRD5A2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx 26kDa band observed in Human Prostate and Breast lysates (calculated MW of 28.4kDa according to NP_000339.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRD5A2

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Long Name

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Alternate Names

5 alpha-SR2, S5AR 2, SR type 2

Entrez Gene IDs

6716 (Human)

Gene Symbol

SRD5A2

Additional SRD5A2 Products

Product Documents for SRD5A2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SRD5A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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