SREBP1 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2215V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Hamster, Plant

Predicted:

Chinese Hamster (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Simple Western, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SREBP1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a portion of the human SREBP1 protein sequence (between residues 300-400).

Reactivity Notes

Use in Human reported in scientific literature (PMID:33842305).

Localization

Membranes of ER, Golgi and Cytoplasmic Vesicles. Processed SREBP1 (1-490 a.a.) translocates to the nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for SREBP1 Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Microarray

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SREBP1

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Long Name

Sterol regulatory element-binding protein 1

Alternate Names

bHLHd1, SREBF1, SREBP-1

Gene Symbol

SREBF1

Additional SREBP1 Products

Product Documents for SREBP1 Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SREBP1 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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