SREBP2 Antibody (1D2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80960

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Chicken

Applications

ELISA, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all SREBP2 Antibodies »

Product Summary for SREBP2 Antibody (1D2) - Azide and BSA Free

Immunogen

Human SREBP2 protein (amino acids 48-403). [UniProt# Q12772]

Reactivity Notes

Chicken was reported in PMIDs 15333705 and 11907029.

Localization

Endoplasmic reticulum and Golgi apparatus membranes,cytoplasmic vesicle. Mature/processed form localized to nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SREBP2 Antibody (1D2) - Azide and BSA Free

Western Blot: SREBP2 Antibody (1D2)Azide and BSA Free [NBP2-80960]

Western Blot: SREBP2 Antibody (1D2)Azide and BSA Free [NBP2-80960]

Western Blot: SREBP2 Antibody (1D2) - Azide and BSA Free [NBP2-80960] - Lane1: HEp-2 cells. Lane 2: MCF-7 cells. Lane 3: LNCaP cells. Lane 4: HT1080 cells. Lane 5: ZR75-1 cells. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody (1D2) - Azide and BSA Free [NBP2-80960]

Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody (1D2) - Azide and BSA Free [NBP2-80960]

Immunocytochemistry/Immunofluorescence: SREBP2 Antibody (1D2) - Azide and BSA Free [NBP2-80960] - SREBP2 (1D2) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.

Applications for SREBP2 Antibody (1D2) - Azide and BSA Free

Application
Recommended Usage

Gel Super Shift Assays

reported in scientific literature (PMID 15333705)

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunoprecipitation

reported in scientific literature (PMID 15213220)

Western Blot

2 - 5 ug/ml
Application Notes
In WB, this antibody is expected to detect the precursor as well as the mature forms of SREBP2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SREBP2

Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. Out of the three isoforms present (SREBP-1a, SREBP-1c and SREBP-2), SREBP-2 has been implicated more directly in the synthesis of cholesterol during metabolism. Similar to SREBP-1a and SREBP-1c, SREBP-2 acts by first binding to sterol regulatory DNA sequences, and subsequently up-regulating the synthesis of enzymes involved in sterol biosynthesis. SREBP-2 is expressed in both the cytoplasm and nucleus on a subcellular level, depending on the state of the protein. It has been known to interact and form a complex with the SCAP protein at the ER, in turn inhibiting interaction between SCAP and SEC24B. This interaction prevents the cleavage of SREBP-2, which results in the synthesis of cholesterol and uptake of LDL despite high levels of present sterols. In recent research, new functions for the SREBP family have offered insight on the effect of lipid metabolism on various pathophysiological diseases (cancers, steatosis and immunity, PMID: 22154484).

Long Name

Sterol Regulatory Element Binding Transcription Factor 2

Alternate Names

bHLhd2, SREBF2

Gene Symbol

SREBF2

Additional SREBP2 Products

Product Documents for SREBP2 Antibody (1D2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SREBP2 Antibody (1D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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