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SREBP2 Antibody (SREBP2/1580)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13783

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SREBP2/1580

Concentration

0.2 mg/ml

Product Summary for SREBP2 Antibody (SREBP2/1580)

Immunogen

Recombinant full-length human SREBP2protein (Uniprot: Q12772)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14186)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SREBP2 Antibody (SREBP2/1580)

Immunohistochemistry-Paraffin: SREBP2 Antibody (SREBP2/1580) [NBP3-13783]

Immunohistochemistry-Paraffin: SREBP2 Antibody (SREBP2/1580) [NBP3-13783]

Immunohistochemistry-Paraffin: SREBP2 Antibody (SREBP2/1580) [NBP3-13783] - IHC analysis of formalin-fixed, paraffin-embedded human Renal carcinoma. Stained using SREBP2 antibody (SREBP2/1580) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Applications for SREBP2 Antibody (SREBP2/1580)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SREBP2

SREBP2 encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.

Long Name

Sterol Regulatory Element Binding Transcription Factor 2

Alternate Names

bHLhd2, SREBF2

Gene Symbol

SREBF2

UniProt

Additional SREBP2 Products

Product Documents for SREBP2 Antibody (SREBP2/1580)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SREBP2 Antibody (SREBP2/1580)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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