SRGAP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49877
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KQEDRQTPRSPDST corresponding to internal region according to NP_056141.2.
Epitope
KQEDRQTPRSPDST - Internal region
Specificity
This antibody is expected to recognize isoform a (NP_056141.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SRGAP2 Antibody
![Western Blot: SRGAP2 Antibody [NBP1-49877]](https://resources.bio-techne.com/images/products/SRGAP2-Antibody-Western-Blot-NBP1-49877-img0002.jpg "Western Blot: SRGAP2 Antibody [NBP1-49877]")
Western Blot: SRGAP2 Antibody [NBP1-49877]
Western Blot: SRGAP2 Antibody [NBP1-49877] - (0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong Univer![Immunocytochemistry/ Immunofluorescence: SRGAP2 Antibody [NBP1-49877]](https://resources.bio-techne.com/images/products/SRGAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49877-img0001.jpg "Immunocytochemistry/ Immunofluorescence: SRGAP2 Antibody [NBP1-49877]")
Immunocytochemistry/ Immunofluorescence: SRGAP2 Antibody [NBP1-49877]
Immunocytochemistry/Immunofluorescence: SRGAP2 Antibody [NBP1-49877] - HEK293 overexpressing Human srGAP2 and probed with EB09775 at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.Applications for SRGAP2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:2000
Peptide ELISA
Detection limit 1:8000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
IF: Transfected HEK293 transiently expressing GFP-tagged human srGAP2 with the primary antibody stained in the red channel (Confocal IF). WB: Approx. 124 kDa band observed in HeLa lysate and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa band according to human NP_056141.2, but the rat protein sequence is stlll provisional).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SRGAP2
Alternate Names
FNBP2, Formin-binding protein 2, KIAA0456ARHGAP34formin binding protein 2, Rho GTPase-activating protein 34, SLIT-ROBO Rho GTPase activating protein 2, SLIT-ROBO Rho GTPase-activating protein 2, srGAP2, SRGAP3
Gene Symbol
SRGAP2
UniProt
Additional SRGAP2 Products
Product Documents for SRGAP2 Antibody
Product Specific Notices for SRGAP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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