SRI Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24479
![Western Blot: SRI AntibodyBSA Free [NBP2-24479]](https://resources.bio-techne.com/images/products/SR1-Antibody-Western-Blot-NBP2-24479-img0002.jpg "Western Blot: SRI AntibodyBSA Free [NBP2-24479]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Canine, Equine, Primate
Cited:
Bovine
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial synthetic peptide made to an internal region of the human SRI protein (between amino acids 45-90) [UniProt P30626]
Reactivity Notes
Xenopus (93%), Opossum (87%), Chicken (81%).
Specificity
100% homologous in human (isoforms CRA_a, CRA_b and CRA_c).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SRI Antibody - BSA Free
Western Blot: SRI AntibodyBSA Free [NBP2-24479]
Western Blot: SR1 Antibody [NBP2-24479] - Analysis of human Sorcin in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using this antibody.![Immunocytochemistry/ Immunofluorescence: SRI Antibody - BSA Free [NBP2-24479]](https://resources.bio-techne.com/images/products/SRI-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24479-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SRI Antibody - BSA Free [NBP2-24479]")
Immunocytochemistry/ Immunofluorescence: SRI Antibody - BSA Free [NBP2-24479]
Immunocytochemistry/Immunofluorescence: SRI Antibody [NBP2-24479] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-SRI at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: SRI Antibody - BSA Free [NBP2-24479]](https://resources.bio-techne.com/images/products/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-24479-img0004.jpg "Immunohistochemistry-Paraffin: SRI Antibody - BSA Free [NBP2-24479]")
Immunohistochemistry-Paraffin: SRI Antibody - BSA Free [NBP2-24479]
Immunohistochemistry-Paraffin: SRI Antibody [NBP2-24479] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of TRIM41 antibody (NBP2-24479). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytosplasmic staing of the glandular cells was observed.Applications for SRI Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-5 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SRI
Alternate Names
calcium binding protein amplified in mutlidrug-resistant cells, CP22, CP-22, FLJ26259,22 kDa protein, H_RG167B05.1, SCN, sorcin, V19
Gene Symbol
SRI
Additional SRI Products
Product Documents for SRI Antibody - BSA Free
Product Specific Notices for SRI Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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