SRI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00310

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SRI Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human SRI (Accession#: P30626; Met1-Val198)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SRI Antibody

Western Blot: SRI Antibody [NBP3-00310]

Western Blot: SRI Antibody [NBP3-00310]

Western Blot: SRI Antibody [NBP3-00310] - Anti-SRI rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 18 kDa
Immunoprecipitation: SRI Antibody [NBP3-00310]

Immunoprecipitation: SRI Antibody [NBP3-00310]

Immunoprecipitation: SRI Antibody [NBP3-00310] - SRI was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 0.5 ul anti-SRI rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SRI rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa

Applications for SRI Antibody

Application
Recommended Usage

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SRI

Sorcin (SRI) is a Ca2+-binding protein first identified in multidrug-resistant cells. This protein is widely distributed in mammalian tissues, including heart and skeletal muscle. At the subcellular level, sorcin localizes to T-tubule junctions of cardiac SR and co-localizes with brain ryanodine receptors in rat brain caudate-putamen nucleus.

Alternate Names

calcium binding protein amplified in mutlidrug-resistant cells, CP22, CP-22, FLJ26259,22 kDa protein, H_RG167B05.1, SCN, sorcin, V19

Gene Symbol

SRI

Additional SRI Products

Product Documents for SRI Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SRI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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