SSEA-1 Antibody (MC-480)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1831

Novus Biologicals, part of Bio-Techne
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NB100-1831
NB100-1831SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chimpanzee

Cited:

Human, Mouse, Chimpanzee

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # MC-480

Concentration

1 mg/ml

View all SSEA-1 Antibodies »

Product Specifications

Immunogen

F9 tetracarcinoma stem cells (X-irradiated).

Reactivity Notes

This antibody recognizes SSEA-1 in mouse EC and ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts). Reactivity with Rat is reported by Kim et al. 2007 (PMID 17548530). Use in Chimpanzee reported in scientific literature (PMID:32045848).

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for SSEA-1 Antibody (MC-480)

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831]

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831]

Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831]

Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831]

Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831] - An intracellular stain was performed on Jurkat cells with SSEA-1 Antibody (MC-480)NB100-1831 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831]

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831]

Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
View 1 more images

Applications for SSEA-1 Antibody (MC-480)

Application
Recommended Usage

Flow Cytometry

2.5 ug/million cells

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Frozen

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Simple Western

1:10

Western Blot

2 ug/ml
Application Notes
This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SSEA-1

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Entrez Gene IDs

2526 (Human)

Gene Symbol

FUT4

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (MC-480)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SSEA-1 Antibody (MC-480)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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