SSEA-3 Antibody (MC-631)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1832

Novus Biologicals, part of Bio-Techne
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NB100-1832
NB100-1832SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Clone # MC-631

Concentration

1 mg/ml

View all SSEA-3 Antibodies »

Product Specifications

Immunogen

4-8 cell stage mouse embryos.

Reactivity Notes

This antibody recognizes the SSEA-3 that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.

Specificity

Recognizes a carbohydrate epitope of SSEA-3

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgM

Scientific Data Images for SSEA-3 Antibody (MC-631)

Immunocytochemistry/ Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832]

Immunocytochemistry/ Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832]

Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - PSC characterisation. Human PSC-1 characterized for typical undifferentiated colony morphology in expression of pluripotency markers Nanog (Catalog # AF1997), OCT-3/4 (Catalog # AF1759), SSEA-3 (NB100-1832), SSEA-4 (Catalog # MAB1435) after immunofluorescence staining. Corresponding nuclei stains with DAPI shown. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-33899-6), licensed under a CC-BY license.
Flow Cytometry: SSEA-3 Antibody (MC-631) [NB100-1832]

Flow Cytometry: SSEA-3 Antibody (MC-631) [NB100-1832]

Flow Cytometry: SSEA-3 Antibody (MC-631) [NB100-1832] - A surface stain was performed on Jurkat cells with SSEA-3 Antibody (MC-631) NB100-1832AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Immunocytochemistry/ Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832]

Immunocytochemistry/ Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832]

Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody.

Applications for SSEA-3 Antibody (MC-631)

Application
Recommended Usage

Flow Cytometry

10-20 ug/ml

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
This SSEA3 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin sections, Immunoprecipitation and Western Blot. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-1832 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SSEA-3

SSEA-3 (stage-specific embryonic antigen-3) is a member of SSEAs which are present on eggs as well as preimplantation embryos at specific stages of early development. SSEAs are glycan epitopes which resides on glycoproteins/glycolipids of mammalian cells, and the epitope of several of these antigens consists solely of sugar residues. SSEA-3 antigen was identified following immunization of rats with 4-8 cell mouse embryos and was present on glycolipids of human teratocarcinoma cells. During protein glycosylation, beta-1,3-galactosyltransferase 5 catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing glycolipid GB5 which is SSEA-3. In its functions. SSEA-3 has been proposed to involve in the process of cell differentiation. SSEA3 expression is mostly restricted to the cytoplasm or apical surface of epithelial cells and it has been reported to express in various normal tissues with high expression levels in kidney. SSEA3 has been reported to be expressed in the ascites fluids from patients of hepatoma or pancreatic cancer, breast cancer including breast cancer stem cells.

Long Name

Stage-specific Embryonic Antigen-3

Alternate Names

SSEA3

Additional SSEA-3 Products

Product Documents for SSEA-3 Antibody (MC-631)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SSEA-3 Antibody (MC-631)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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