Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine, Chicken, Primate, Rabbit

Cited:

Human, Rabbit

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # MC-813-70

Concentration

1.0 mg/ml

View all SSEA-4 Antibodies »

Product Specifications

Immunogen

Human embryonal carcinoma cell line 2102Ep

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for SSEA-4 Antibody (MC-813-70)

Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644]

Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644]

Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Analysis using the FITC conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26645 (green histogram represents isotype control; red represents SSEA-4 antibody).
Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644]

Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644]

Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Cell surface analysis on 2102Ep cells using 0.1 ug/10^6 cells of NBP2-26644 (green histogram represents isotype control; red represents SSEA-4 antibody).
Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644]

Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644]

Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Cell surface flow cytometric analysis of SSEA-4 PE on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26648 (green histogram represents isotype control; red represents SSEA-4 antibody).
View 1 more images

Applications for SSEA-4 Antibody (MC-813-70)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 28859517)

Western Blot

reported in scientific literature (PMID 28302994)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SSEA-4

SSEA-4 specific antibody, clone MC-813, is reactive with Stage-Specific Embyronic Antigen-4, a carbohydrate antigen expressed on human teratocarcinoma cells (EC), embryonic stem cells (ES) and embryonic germ cells. It is also expressed on a subset of mesenchymal stem cells, erythrocytes, the erythroleukemic myeloid cell line K562, unfertilized oocytes and early cleaved stage oocytes. This antibody was first categorized in the following publication: PMID 6141938 https://pubmed.ncbi.nlm.nih.gov/6141938/

Long Name

Stage-specific Embryonic Antigen-4

Alternate Names

SSEA4

Additional SSEA-4 Products

Product Documents for SSEA-4 Antibody (MC-813-70)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SSEA-4 Antibody (MC-813-70)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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