SSH3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60675

Novus Biologicals, part of Bio-Techne
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NB100-60675
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SSH3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 609 and 659 of human slingshot homolog 3 using the numbering given in entry NP_060327.2 (GeneID 54961).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SSH3 Antibody

Western Blot: SSH3 Antibody [NB100-60675]

Western Blot: SSH3 Antibody [NB100-60675]

Western Blot: SSH3 Antibody [NB100-60675] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60675 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SSH3 was also immunoprecipitated by rabbit anti-SSH3 antibody NB100-60674, which recognizes an upstream epitope.
Immunohistochemistry: SSH3 Antibody [NB100-60675]

Immunohistochemistry: SSH3 Antibody [NB100-60675]

Immunohistochemistry: SSH3 Antibody [NB100-60675] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SSH3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SSH3 Antibody [NB100-60675]

Immunohistochemistry-Paraffin: SSH3 Antibody [NB100-60675]

Immunohistochemistry-Paraffin: SSH3 Antibody [NB100-60675] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-SSH3 used at a dilution of 1:250.

Applications for SSH3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SSH3

SSH3, also known as slingshot-3, may function as a phosphatase that activates the actin severing protein, ADF/cofilin to indfluence actin dynamics. There are three members of the slingshot family, SSH1, SSH2, and SSH3.

Alternate Names

EC 3.1.3.16, EC 3.1.3.48, FLJ10928, FLJ20515, FLJ42240, hSSH-3L, protein phosphatase Slingshot homolog 3, slingshot 3, slingshot homolog 3 (Drosophila), SSH3, SSH3L, SSH-3L, SSH-like protein 3

Entrez Gene IDs

54961 (Human)

Gene Symbol

SSH3

UniProt

NP_060327.2 (Human)

Additional SSH3 Products

Product Documents for SSH3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SSH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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