SSRP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33235

Novus Biologicals, part of Bio-Techne
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NBP1-33235
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (99%), Mouse (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SSRP1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SSRP1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSRP1 Antibody

Western Blot: SSRP1 Antibody [NBP1-33235]

Western Blot: SSRP1 Antibody [NBP1-33235]

Western Blot: SSRP1 Antibody [NBP1-33235] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SSRP1 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-33235]

Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-33235]

Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-33235] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.
Western Blot: SSRP1 Antibody [NBP1-33235]

Western Blot: SSRP1 Antibody [NBP1-33235]

Western Blot: SSRP1 Antibody [NBP1-33235] - (30 ug of whole cell lysate) Molt-4 7.5% SDS PAGE; antibody diluted at 1:1000
View 1 more images

Applications for SSRP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IP-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SSRP1

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D7 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Alternate Names

Chromatin-specific transcription elongation factor 80 kDa subunit, cisplatin-DNA SSRP, facilitates chromatin remodeling 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT, FACT complex subunit SSRP1, FACT80FACT 80 kDa subunit, FACTp80, high mobility group box, hSSRP1, Recombination signal sequence recognition protein 1, structure specific recognition protein 1, Structure-specific recognition protein 1, T160

Gene Symbol

SSRP1

Additional SSRP1 Products

Product Documents for SSRP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SSRP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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