ST3GAL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20492

Novus Biologicals, part of Bio-Techne
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NBP2-20492
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (94%), Chimpanzee (98%), Rat (90%), Rhesus Macaque (98%), Sheep (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ST3GAL5/GM3 Synthase Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ST3GAL5. The exact sequence is proprietary.

Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ST3GAL5 Antibody

Western Blot: ST3GAL5 Antibody [NBP2-20492]

Western Blot: ST3GAL5 Antibody [NBP2-20492]

Western Blot: ST3GAL5 Antibody [NBP2-20492] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: ST3GAL5 Antibody [NBP2-20492]

Immunohistochemistry-Paraffin: ST3GAL5 Antibody [NBP2-20492]

Immunohistochemistry-Paraffin: ST3GAL5 Antibody [NBP2-20492] - Human breast cancer, using ST3GAL5 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: ST3GAL5 Antibody [NBP2-20492]

Western Blot: ST3GAL5 Antibody [NBP2-20492]

Western Blot: ST3GAL5 Antibody [NBP2-20492] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.
View 1 more images

Applications for ST3GAL5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ST3GAL5/GM3 Synthase

Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 5

Alternate Names

3S-T, Ganglioside GM3 Synthase, SATI, Siat9, ST3GALV

Gene Symbol

ST3GAL5

UniProt

Q9UNP4 (Human)

Additional ST3GAL5/GM3 Synthase Products

Product Documents for ST3GAL5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ST3GAL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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