ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47890

Novus Biologicals, part of Bio-Techne
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NBP2-47890-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # LN-1

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all ST6 Gal Sialyltransferase 1/ST6GAL1 Antibodies »

Product Specifications

Immunogen

Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

Localization

Cell surface and cytoplasmic

Specificity

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This monoclonal antibody reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44669).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Immunocytochemistry/ Immunofluorescence: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890]

Immunocytochemistry/ Immunofluorescence: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890]

Immunocytochemistry/Immunofluorescence: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeled with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890] - Formalin-fixed, paraffin-embedded normal human spleen stained with CDw75 monoclonal antibody (LN-1).
Flow Cytometry: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890]

Flow Cytometry: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890]

Flow Cytometry: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (LN-1) - Azide and BSA Free [NBP2-47890] - Surface staining of human lymphocytes with CDw75 Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.

Applications

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

PEG purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

CD75 is encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. This antibody recognizes the CD75 cell surface antigen, an alpha 2,6 sialyated carbohydrate epitope expressed by mature B cells, especially germinal centre B cells, red blood cells and some epithelial cells.

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1

Alternate Names

Alpha 2,6-ST, B-cell antigen CD75, CD75, SIAT1, ST6N

Gene Symbol

ST6GAL1

Additional ST6 Gal Sialyltransferase 1/ST6GAL1 Products

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Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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