STAG2 Antibody (1417)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43823

Novus Biologicals, part of Bio-Techne
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NBP2-43823
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rabbit (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1417

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all STAG2 Antibodies »

Product Summary for STAG2 Antibody (1417)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STAG2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAG2 Antibody (1417)

Western Blot: STAG2 Antibody (1417) [NBP2-43823]

Western Blot: STAG2 Antibody (1417) [NBP2-43823]

Western Blot: STAG2 Antibody (1417) [NBP2-43823] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody [1417]. The signal was developed with Trident ECL plus-Enhanced.
Western Blot: STAG2 Antibody (1417) [NBP2-43823]

Western Blot: STAG2 Antibody (1417) [NBP2-43823]

Western Blot: STAG2 Antibody (1417) [NBP2-43823] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG2 antibody diluted at a dilution of 1:1000.

Applications for STAG2 Antibody (1417)

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STAG2

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Alternate Names

bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753

Gene Symbol

STAG2

Additional STAG2 Products

Product Documents for STAG2 Antibody (1417)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAG2 Antibody (1417)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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