STAG2 Antibody (3H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15155

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15155
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3H4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all STAG2 Antibodies »

Product Summary for STAG2 Antibody (3H4)

Immunogen

A synthesized peptide derived from human STAG2. UniProt ID (Q8N3U4).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAG2 Antibody (3H4)

Western Blot: STAG2 Antibody (3H4) [NBP3-15155]

Western Blot: STAG2 Antibody (3H4) [NBP3-15155]

Western Blot: STAG2 Antibody (3H4) [NBP3-15155] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: STAG2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 142, 146 KDa. Observed band size: 142 KDa.
Immunocytochemistry/ Immunofluorescence: STAG2 Antibody (3H4) [NBP3-15155]

Immunocytochemistry/ Immunofluorescence: STAG2 Antibody (3H4) [NBP3-15155]

Immunocytochemistry/Immunofluorescence: STAG2 Antibody (3H4) [NBP3-15155] - Immunofluorescence staining of MCF7 Cells with at STAG2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for STAG2 Antibody (3H4)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: STAG2

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Alternate Names

bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753

Gene Symbol

STAG2

Additional STAG2 Products

Product Documents for STAG2 Antibody (3H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAG2 Antibody (3H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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