STAG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60946

Novus Biologicals, part of Bio-Techne
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NB100-60946
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all STAG2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-REPKRLRPEDS corresponding to internal region according to NP_001036214.1, NP_001036215.1 , NP_001036216.1 , NP_006594.3.

Epitope

REPKRLRPEDS

Specificity

This antibody is expected to recognise isoform a (NP_001036214.1 and NP_001036215.1) and isoform b (NP_001036216.1 and NP_006594.3).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for STAG2 Antibody

Western Blot: STAG2 Antibody [NB100-60946]

Western Blot: STAG2 Antibody [NB100-60946]

Western Blot: STAG2 Antibody [NB100-60946] - Analysis of STAG2 using this antibody at 0.01 ug/ml in HeLa cell lysate (35ug protein in RIPA buffer).

Applications for STAG2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in HeLa lysates (calculated MW of 146 kDa band according to NP_001036214.1 and NP_001036215.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAG2

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Alternate Names

bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753

Entrez Gene IDs

10735 (Human); 20843 (Mouse)

Gene Symbol

STAG2

UniProt

NP_001036214.1 (Human)

Additional STAG2 Products

Product Documents for STAG2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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