STAM-1 Antibody (29C678)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56533

Novus Biologicals, part of Bio-Techne
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NB100-56533
NB100-56533SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 29C678

Concentration

0.5 mg/ml

View all STAM-1 Antibodies »

Product Summary for STAM-1 Antibody (29C678)

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 149-165 of human STAM.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for STAM-1 Antibody (29C678)

Western Blot: STAM-1 Antibody (29C678) [NB100-56533]

Western Blot: STAM-1 Antibody (29C678) [NB100-56533]

Western Blot: STAM-1 Antibody (29C678) [NB100-56533] - Western blot analysis of HeLa lysate (35ug per lane, RIPA buffer) using STAM antibody (NB100-56533) at 1ug/ml. Band observed at ~65kDA. (Expected MW of 59.2kDa according to NP_003464.1).

Applications for STAM-1 Antibody (29C678)

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
A 59 kDa band is observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAM-1

STAM is a signal transducing adaptor molecule, that contains an Src homology 3 (SH3) domain and immunoreceptor tyrosine-based activation motif (ITAM). STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF, respectively. An SH3 deletion mutant of STAM confers a dominant-negative effect on DNA synthesis mediated by IL-2 and GM-CSF. Furthermore, the wild-type STAM, but not STAM mutants deleted of SH3 and ITAM, significantly enhances c-myc induction mediated by IL-2 and GM-CSF. These results strongly implicate STAM in the signaling pathways for cell growth and c-myc induction immediately downstream of the Jaks associated with the cytokine receptors.

Long Name

Signal Transducing Adaptor Molecule 1

Alternate Names

HSE1 Homolog, STAM, STAM1

Gene Symbol

STAM

UniProt

Q92783 (Human)

Additional STAM-1 Products

Product Documents for STAM-1 Antibody (29C678)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAM-1 Antibody (29C678)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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