STAP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88586

Novus Biologicals, part of Bio-Techne
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NBP1-88586
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all STAP2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NYFVSHTKKALVPFLLDEDYEKVLGYVEADKENGENVWVAPSAPGPGPAPCTGGPKPLSPASSQDKLPPLPPLPNQEENYVTPIGDGPAVDYENQDVASSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAP2 Antibody

Western Blot: STAP2 Antibody [NBP1-88586]

Western Blot: STAP2 Antibody [NBP1-88586]

Western Blot: STAP2 Antibody [NBP1-88586] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunohistochemistry-Paraffin: STAP2 Antibody [NBP1-88586]

Immunohistochemistry-Paraffin: STAP2 Antibody [NBP1-88586]

Immunohistochemistry-Paraffin: STAP2 Antibody [NBP1-88586] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Western Blot: STAP2 Antibody [NBP1-88586]

Western Blot: STAP2 Antibody [NBP1-88586]

Western Blot: STAP2 Antibody [NBP1-88586] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4

Applications for STAP2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAP2

STAP2 encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants.

Alternate Names

BKSbrk kinase substrate, Breast tumor kinase substrate, BRK substrate, FLJ20234, signal transducing adaptor family member 2, signal-transducing adaptor protein 2, signal-transducing adaptor protein-2, STAP-2

Gene Symbol

STAP2

Additional STAP2 Products

Product Documents for STAP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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