Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI15H3

Concentration

0.6 mg/ml

View all STAT1 Antibodies »

Product Summary for STAT1 Antibody (OTI15H3)

Immunogen

Full-length protein expressed in 293T cell transfected with human STAT1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT1 Antibody (OTI15H3)

Western Blot: STAT1 Antibody (OTI15H3) [NBP1-48013]

Western Blot: STAT1 Antibody (OTI15H3) [NBP1-48013]

Western Blot: STAT1 Antibody (15H3) [NBP1-48013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.
Immunocytochemistry/Immunofluorescence: STAT1 Antibody (15H3) [NBP1-48013] Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.
Immunohistochemistry-Paraffin: STAT1 Antibody (OTI15H3) [NBP1-48013]

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI15H3) [NBP1-48013]

Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STAT1 mouse monoclonal antibody.
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Applications for STAT1 Antibody (OTI15H3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT1

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human)

Gene Symbol

STAT1

Additional STAT1 Products

Product Documents for STAT1 Antibody (OTI15H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for STAT1 Antibody (OTI15H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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