STAT1 Antibody (SM1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-514
![Immunoprecipitation: STAT1 Antibody (SM1) - BSA Free [NB500-514]](https://resources.bio-techne.com/images/products/STAT1-Antibody-SM1-Immunoprecipitation-NB500-514-img0001.jpg "Immunoprecipitation: STAT1 Antibody (SM1) - BSA Free [NB500-514]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # SM1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for STAT1 Antibody (SM1) - BSA Free
Immunoprecipitation: STAT1 Antibody (SM1) - BSA Free [NB500-514]
Immunoprecipitation: STAT1 Antibody (SM1) [NB500-514] - Fig. 1: Induction of phosphorylation of STAT1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT1 (Ser727) by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).Applications for STAT1 Antibody (SM1) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Immunoprecipitation
1:50
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (paraffin sections) positive tissue: lung macrophages.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: STAT1
Long Name
Signal Transducer and Activator of Transcription 1
Alternate Names
CANDF7, ISGF-3, STAT91
Gene Symbol
STAT1
UniProt
Additional STAT1 Products
Product Documents for STAT1 Antibody (SM1) - BSA Free
Product Specific Notices for STAT1 Antibody (SM1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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