STAT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56314
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine, Canine, Primate
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
A synthetic peptide sequence corresponding to amino acids between 700 and 750 of human Stat1 was used as immunogen. This sequence is 100% conserved between Stat1 alpha and Stat1 beta.
Specificity
This antibody is able to detect both the alpha form (p91, ~91 kD) and the beta form (p84, ~84 kD) of STAT1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for STAT1 Antibody
![Western Blot: STAT1 Antibody [NB100-56314]](https://resources.bio-techne.com/images/products/STAT1-Antibody-Western-Blot-NB100-56314-img0002.jpg "Western Blot: STAT1 Antibody [NB100-56314]")
Western Blot: STAT1 Antibody [NB100-56314]
Western Blot: STAT1 Antibody [NB100-56314] - Western blot analysis of STAT1 in HeLa cell lysate using NB100-56314.![Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]](https://resources.bio-techne.com/images/products/STAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56314-img0004.jpg "Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]")
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]
Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NB100-56314] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-STAT1 Antibody NB100-56314 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.![Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]](https://resources.bio-techne.com/images/products/STAT1-Antibody-Immunohistochemistry-Paraffin-NB100-56314-img0003.jpg "Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]")
Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]
Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of STAT1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong nuclear staining in germinal center and surrounding cells along with cytoplasmic reactivity was observed.Applications for STAT1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-5 ug/ml
Western Blot
1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: STAT1
Long Name
Signal Transducer and Activator of Transcription 1
Alternate Names
CANDF7, ISGF-3, STAT91
Entrez Gene IDs
20846 (Mouse)
Gene Symbol
STAT1
UniProt
Additional STAT1 Products
Product Documents for STAT1 Antibody
Product Specific Notices for STAT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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