STAT3 Antibody (3B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51667

Novus Biologicals, part of Bio-Techne
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NBP1-51667-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Monkey, Primate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B5

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all STAT3 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human STAT3 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT3 Antibody (3B5)

Western Blot: STAT3 Antibody (3B5)BSA Free [NBP1-51667]

Western Blot: STAT3 Antibody (3B5)BSA Free [NBP1-51667]

Western Blot: STAT3 Antibody (3B5) [NBP1-51667] - Analysis using STAT3 mouse mAb against Hela (1),NIH/3T3 (2), Jurket (3), PC-12 (4) and COS7 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: STAT3 Antibody (3B5) - BSA Free [NBP1-51667]

Immunocytochemistry/ Immunofluorescence: STAT3 Antibody (3B5) - BSA Free [NBP1-51667]

Immunocytochemistry/Immunofluorescence: STAT3 Antibody (3B5) [NBP1-51667] - Analysis of Hela cells using STAT3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: STAT3 Antibody (3B5) - BSA Free [NBP1-51667]

Immunohistochemistry-Paraffin: STAT3 Antibody (3B5) - BSA Free [NBP1-51667]

Immunohistochemistry-Paraffin: STAT3 Antibody (3B5) [NBP1-51667] - Analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) using STAT3 mouse mAb with DAB staining.

Applications for STAT3 Antibody (3B5)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT3

STAT 3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT 3 induces nuclear translocation to activate transcription. STAT3 is expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. STAT 3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT 3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT 3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT 3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by Rac1 protein. STAT 3 interacts with Rac1, STAT 1, and NCOA1 proteins. The Poly6246 antibody recognizes the C-terminal region of human, mouse, and rat STAT3 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry. The Poly6246 antibody does not react with STAT3b. Interacts with Rac1, associates with STAT1, NCOA1

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

Additional STAT3 Products

Product Documents for STAT3 Antibody (3B5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAT3 Antibody (3B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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