STAT3 Antibody (CL0492) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43941

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0492

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all STAT3 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P40763, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for STAT3 Antibody (CL0492) - Azide and BSA Free

STAT3 Antibody (CL0492) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: STAT3 Antibody [NBP3-43941]

Immunocytochemistry/ Immunofluorescence: STAT3 Antibody [NBP3-43941]

Staining of A549 cells using the Anti-STAT3 monoclonal antibody) .
STAT3 Antibody (CL0492) - Azide and BSA Free Immunohistochemistry-Paraffin: STAT3 Antibody [NBP3-43941]

Immunohistochemistry-Paraffin: STAT3 Antibody [NBP3-43941]

Analysis in human endometrium and pancreas tissues using NBP3-43941 antibody. Corresponding STAT3 RNA-seq data are presented for the same tissues.
STAT3 Antibody (CL0492) - Azide and BSA Free Western Blot: STAT3 Antibody [NBP3-43941]

Western Blot: STAT3 Antibody [NBP3-43941]

Lane 1: Marker [kDa]
Lane 2: Human cell line RT-4
View 5 more images

Applications for STAT3 Antibody (CL0492) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

Additional STAT3 Products

Product Documents for STAT3 Antibody (CL0492) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAT3 Antibody (CL0492) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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