STAT3 [p Tyr705] Antibody (RM261)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61588

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-61588
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM261

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all STAT3 Antibodies »

Product Specifications

Immunogen

A phospho-peptide corresponding to Human STAT3 [p Tyr705]

Reactivity Notes

This antibody may also react to mouse or rat phospho-STAT3 [Tyr705], as predicted by immunogen homology.

Modification

p Tyr705

Specificity

This antibody reacts to human STAT3 only when phosphorylated at Tyr705. There is no cross-reactivity to STAT3 without phosphorylation at Tyr705.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAT3 [p Tyr705] Antibody (RM261)

Western Blot: STAT3 [p Tyr705] Antibody (RM261) [NBP2-61588] - Western Blot of A431 cell lysates, nontreated (-) or treated (+) with EGF, using NBP2-61588, showed Phospho-Stat3 (Tyr705) in EGF-treated A431 cells.
Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody (RM261) [NBP2-61588] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61588.
Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody (RM261) [NBP2-61588] - Human intestine. Image from verified customer review.

Applications for STAT3 [p Tyr705] Antibody (RM261)

Application
Recommended Usage

Immunohistochemistry

1:1000-1:10000

Immunohistochemistry-Paraffin

1:1000-1:10000

Western Blot

1:1000 - 1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-61588 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

Additional STAT3 Products

Product Documents for STAT3 [p Tyr705] Antibody (RM261)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAT3 [p Tyr705] Antibody (RM261)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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