STAT3 [p Tyr705] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82213

Novus Biologicals, part of Bio-Techne
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NB100-82213
NB100-82213-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all STAT3 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of tyrosine 705 (A-P-YP-L-K).

Modification

p Tyr705

Specificity

Detects endogenous levels of STAT3 only when phosphorylated at tyrosine 705.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT3 [p Tyr705] Antibody

Western Blot: STAT3 [p Tyr705] Antibody [NB100-82213]

Western Blot: STAT3 [p Tyr705] Antibody [NB100-82213]

Western Blot: STAT3 [p Tyr705] Antibody [NB100-82213] - Analysis of extracts from Hela cells untreated or treated with IFN a using STAT3
Immunocytochemistry/ Immunofluorescence: STAT3 [p Tyr705] Antibody [NB100-82213]

Immunocytochemistry/ Immunofluorescence: STAT3 [p Tyr705] Antibody [NB100-82213]

Immunocytochemistry/Immunofluorescence: STAT3 [p Tyr705] Antibody [NB100-82213] - Sstaining of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody.
Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213]

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213]

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of STAT3 (Tyr705) in paraffin-embedded human breast carcinoma tissue using NB100-82213.
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Applications for STAT3 [p Tyr705] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-200

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50 - 1:100

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:500-1:2000
Application Notes
This STAT3 [p Tyr705] Antibody is validated for Simple Western from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-82213 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT3

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Gene Symbol

STAT3

UniProt

P40763

Additional STAT3 Products

Product Documents for STAT3 [p Tyr705] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAT3 [p Tyr705] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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