STAT5a/b Antibody (SR2282)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21692
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR2282
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for STAT5a/b Antibody (SR2282)
Immunogen
A synthesized peptide derived from human STAT5a/b (Uniprot #: P42229, P51692 )
Specificity
Detects endogenous levels of STAT5a/b
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for STAT5a/b Antibody (SR2282)
Immunohistochemistry: STAT5a/b Antibody (SR2282) [NBP3-21692] -
Immunohistochemistry: STAT5a/b Antibody (SR2282) [NBP3-21692] - Immunohistochemical analysis of paraffin-embedded human breast, using NBP3-21692.
Western Blot: STAT5a/b Antibody (SR2282) [NBP3-21692] -
Western Blot: STAT5a/b Antibody (SR2282) [NBP3-21692] - Western blot analysis using NBP3-21692 in (1) Jurkat cell lysate; (2) 3T3 cell lysate; (2) PC12 cell lysate.Applications for STAT5a/b Antibody (SR2282)
Application
Recommended Usage
Flow Cytometry
1:50
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:100-1:500
Immunoprecipitation
1:50
Western Blot
1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: STAT5a/b
Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.
Long Name
Signal Transducer and Activator of Transcription 5b
Alternate Names
MGF, STAT5, STAT5A signal transducer and activator of transcription 5A
Gene Symbol
STAT5A
Additional STAT5a/b Products
Product Documents for STAT5a/b Antibody (SR2282)
Product Specific Notices for STAT5a/b Antibody (SR2282)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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