STAT5b Antibody (6P5T0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15778

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15778-100ul
NBP3-15778-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6P5T0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all STAT5b Antibodies »

Product Summary for STAT5b Antibody (6P5T0)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 600-787 of human STAT5B (P51692). KQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAT5b Antibody (6P5T0)

Western Blot: STAT5b Antibody (6P5T0) [NBP3-15778]

Western Blot: STAT5b Antibody (6P5T0) [NBP3-15778]

Western Blot: STAT5b Antibody (6P5T0) [NBP3-15778] - Western blot analysis of extracts of various cell lines, using STAT5b antibody (NBP3-15778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Knockout Validated: STAT5b Antibody (6P5T0) [NBP3-15778]

Knockout Validated: STAT5b Antibody (6P5T0) [NBP3-15778]

Knockout Validated: STAT5b Antibody (6P5T0) [NBP3-15778] - Western blot analysis of extracts from normal (control) and STAT5b knockout (KO) HeLa cells, using STAT5b antibody (NBP3-15778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: STAT5b Antibody (6P5T0) [NBP3-15778]

Immunoprecipitation: STAT5b Antibody (6P5T0) [NBP3-15778]

Immunoprecipitation: STAT5b Antibody (6P5T0) [NBP3-15778] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug STAT5b antibody (NBP3-15778). Western blot was performed from the immunoprecipitate using STAT5b antibody (NBP3-15778) at a dilition of 1:1000.

Applications for STAT5b Antibody (6P5T0)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT5b

STAT5B is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

signal transducer and activator of transcription 5B, STAT5, transcription factor STAT5B

Gene Symbol

STAT5B

Additional STAT5b Products

Product Documents for STAT5b Antibody (6P5T0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAT5b Antibody (6P5T0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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