Stathmin 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76798

Novus Biologicals, part of Bio-Techne
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NBP1-76798
NBP1-76798SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chinese Hamster (100%), Porcine (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Stathmin/STMN1 Antibodies »

Product Summary for Stathmin 1 Antibody - BSA Free

Immunogen

A synthetic peptide made to an C-terminal portion of the human Stathmin 1 protein (between residues 100-149) [UniProt P16949]

Reactivity Notes

Predicted to react in bovine, primate, pig, Chinese Hamster and canine based on 100% sequence identity.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Stathmin 1 Antibody - BSA Free

Knockout Validated: Stathmin 1 Antibody - BSA Free [NBP1-76798]

Knockout Validated: Stathmin 1 Antibody - BSA Free [NBP1-76798]

Knockout Validated: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Stathmin 1 AntibodyBSA Free [NBP1-76798]

Western Blot: Stathmin 1 AntibodyBSA Free [NBP1-76798]

Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Stathmin 1 Antibody - BSA Free [NBP1-76798]

Immunocytochemistry/ Immunofluorescence: Stathmin 1 Antibody - BSA Free [NBP1-76798]

Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Applications for Stathmin 1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:100 - 1:250

Immunohistochemistry-Paraffin

1:100 - 1:250

Western Blot

0.5-1 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Stathmin/STMN1

Stathmin 1 (STMN1), also known as leukemia-associated phosphoprotein p18, metablastin, oncoprotein 18 (op18), phosphoprotein p19, prosolin, protein Pr22, and pp17, is a member of the stathmin family that encodes a ubiquitous cytosolic phosphoprotein that functions as an intracellular relay for regulatory signals of the cellular environment. STMN1 is involved in the regulation of the microtubule filament system by destabilizing microtubules. In addition, STMN1 has resently been implicated as a potential target of the ASK1-p38 MAP kinase cascade. Phosphorylation at STMN1 Ser-16 may be required for axon formation during neurogenesis. The STMN1 gene has been linked to the control of the learned and innate fear responses. STMN1 is highly expressed in a wide variety of human malignancies, including leukemia, prostate cancer, ovarian carcinoma, and breast carcinoma; suggesting that STMN1 may be an ideal target for anti-cancer therapeutics.

Alternate Names

LAP18, Metablastin, OP18, Phosphoprotein p19, Prosolin, SMN, STMN1

Entrez Gene IDs

3925 (Human); 16765 (Mouse)

Gene Symbol

STMN1

UniProt

P16949

Additional Stathmin/STMN1 Products

Product Documents for Stathmin 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Stathmin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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