Stathmin-2/STMN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49461

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse, Rat, Avian - Chicken

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, In vivo assay, Knockdown Validated, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Stathmin-2/STMN2 Antibodies »

Product Specifications

Immunogen

C-terminal peptide of mouse STMN2. [Swiss-Prot P55821]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32778834).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Stathmin-2/STMN2 Antibody

Knockdown Validation of Stathmin-2/STMN2 Antibody in Mouse DRG Neurons by Western Blot

Knockdown Validation of Stathmin-2/STMN2 Antibody in Mouse DRG Neurons by Western Blot

Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons. A partial siRNA knockdown was used in the second lane. NBP1-49461 was used at a dilution of 1:1000. Image courtesy of Dr. Jung Eun Shin.
Immunocytochemistry/Immunofluorescence Analysis of Stathmin-2/STMN2 in Mouse DRG Neurons

Immunocytochemistry/Immunofluorescence Analysis of Stathmin-2/STMN2 in Mouse DRG Neurons

Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons Image shows the expected staining of endogenous STMN2 in axons and growth cones. Tubulin is a marker of axons. NBP1-49461 was used at a dilution of 1:4000. Image courtesy of Dr. Jung Eun Shin.
Immunohistochemical Staining of Stathmin-2/STMN2 in Frozen Mouse Sciatic Nerves

Immunohistochemical Staining of Stathmin-2/STMN2 in Frozen Mouse Sciatic Nerves

Neuronal deletion of Tsc2 improves axon regeneration within the first 2 d following nerve injury. SCG10 immunostaining of sciatic nerves 1 d after crush injury in control and Tsc2 cKO mice with (conditioned) or without (sham) a conditioning injury. Red dotted line denotes the crush site while red arrowheads point to the three longest axons. Scale bar: 500 i1/4m. Image collected and cropped by Citeab from the following publication (Nociceptor Deletion of Tsc2 Enhances Axon Regeneration by Inducing a Conditioning Injury Response in Dorsal Root Ganglia. Eneuro (2019) licensed under a CC-BY license.
View 3 more images

Applications for Stathmin-2/STMN2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 35042776)

Immunohistochemistry-Frozen

1:200 - 1:500

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 31182472)

In vitro assay

reported in scientific literature (PMID 22726832)

In vivo assay

reported in scientific literature (PMID 22726832)

Western Blot

1 - 2 ug/ml
Application Notes
In Western blot a band can be seen at ~22 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-49461 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Stathmin-2/STMN2

STMN2 (Stathmin-2 or SCG10/Scgn10) belongs to stathmin family of proteins which are known to bind tubulin for acting as a sequestering agents and for promoting microtubule disassembly. STMN2 is a membrane-associated neuronal protein expressed mainly during development and its expression correlates with neurite outgrowth. STMN2 possesses a unique N-terminal domain that is critical for membrane binding, is responsible for STMN2 localization to the Golgi complex and is important in its targeting to growth cones. STMN2 localize to perinuclear cytoplasm, axons, and growth cones of developing neurons, and exists in soluble as well as membrane-bound forms. STMN2 interacts with ITMC2, as well as MAPK8, and when phosphorylated by MAPK8, it act as regulator of microtubule stability which controls neurite length in cortical neurons. Unlike other stathmins, STMN2 acts in two ways to promote microtubule dynamics: it stabilizes microtubules at their plus end and promotes microtubule catastrophe at their minus end. In the developing brain, STMN2 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Reduced STMN2 expression is associated with Down's syndrome and Alzheimer's disease.

Alternate Names

SCG10, SCGN10, Stathmin2, STMN2

Gene Symbol

STMN2

Additional Stathmin-2/STMN2 Products

Product Documents for Stathmin-2/STMN2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Stathmin-2/STMN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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