STI1 Antibody (1E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00010963-M11

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all STI1 Antibodies »

Product Specifications

Immunogen

STIP1 (NP_006810.1, 445 a.a. ~ 543 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR

Reactivity Notes

Human, Mouse & Rat. Other species have not been tested.

Specificity

STIP1 (1E3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for STI1 Antibody (1E3)

Western Blot: STI1 Antibody (1E3) [H00010963-M11]

Western Blot: STI1 Antibody (1E3) [H00010963-M11]

Western Blot: STI1 Antibody (1E3) [H00010963-M11] - Analysis of STIP1 expression in Raw 264.7 (Cat # L024V1).
Immunocytochemistry/ Immunofluorescence: STI1 Antibody (1E3) [H00010963-M11]

Immunocytochemistry/ Immunofluorescence: STI1 Antibody (1E3) [H00010963-M11]

Immunocytochemistry/Immunofluorescence: STI1 Antibody (1E3) [H00010963-M11] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: STI1 Antibody (1E3) [H00010963-M11]

Immunohistochemistry-Paraffin: STI1 Antibody (1E3) [H00010963-M11]

Immunohistochemistry-Paraffin: STI1 Antibody (1E3) [H00010963-M11] - Analysis of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml
View 4 more images

Applications for STI1 Antibody (1E3)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STI1

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 (PubMed 16100115)).(supplied by OMIM)

Long Name

Stress-induced Phosphoprotein 1

Alternate Names

HOP, IEF-SSP-3521, NY-REN-11, STI1L, STIP1

Entrez Gene IDs

10963 (Human)

Gene Symbol

STIP1

OMIM

605063 (Human)

UniProt

NP_006810 (Human)

Additional STI1 Products

Product Documents for STI1 Antibody (1E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for STI1 Antibody (1E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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