Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CDN3H4

Format

BSA Free

Concentration

1.0 mg/ml

View all STIM1 Antibodies »

Product Specifications

Immunogen

Synthetized peptide from C-terminal cytoplasmic part of STIM1. (NM_003156.3).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STIM1 Antibody (CDN3H4) - BSA Free

Western Blot: STIM1 Antibody (CDN3H4)BSA Free [NB110-55300]

Western Blot: STIM1 Antibody (CDN3H4)BSA Free [NB110-55300]

Western Blot: STIM1 Antibody (CDN3H4) [NB110-55300] - (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)
Immunocytochemistry/ Immunofluorescence: STIM1 Antibody (CDN3H4) - BSA Free [NB110-55300]

Immunocytochemistry/ Immunofluorescence: STIM1 Antibody (CDN3H4) - BSA Free [NB110-55300]

Immunocytochemistry/Immunofluorescence: STIM1 Antibody (CDN3H4) [NB110-55300] - Staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor(R) 598; red). Nuclei were stained with DAPI (blue).

Applications for STIM1 Antibody (CDN3H4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:50

Western Blot

1 ug/ml
Application Notes
Western Blotting - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions. Immunocytochemistry - Staining technique: methanol-aceton fixation

Reviewed Applications

Read 1 review rated 4 using NB110-55300 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: STIM1

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Entrez Gene IDs

6786 (Human); 20866 (Mouse); 361618 (Rat)

Gene Symbol

STIM1

UniProt

Q13586

Additional STIM1 Products

Product Documents for STIM1 Antibody (CDN3H4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STIM1 Antibody (CDN3H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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