STIM1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60547

Novus Biologicals, part of Bio-Techne
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NB110-60547
NB110-60547SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine

Cited:

Human, Rat

Predicted:

Porcine (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all STIM1 Antibodies »

Product Specifications

Immunogen

STIM1 antibody was made to a synthetic peptide from the C-terminal region (within residues 600-685) of the human STIM1 protein. [UniProt# Q13586]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: porcine (93%) and Xenopus (79%).

Localization

Cell membrane, ER membrane. Translocates from ER to cell membrane in response to depletion of intracellular calcium.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STIM1 Antibody - BSA Free

Knockdown Validated: STIM1 Antibody - BSA Free [NB110-60547]

Knockdown Validated: STIM1 Antibody - BSA Free [NB110-60547]

Knockdown Validated: STIM1 Antibody [NB110-60547] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stim-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Stim-1 Polyclonal Antibody (Catalog # NB110-60547) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stim-1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: STIM1 AntibodyBSA Free [NB110-60547]

Western Blot: STIM1 AntibodyBSA Free [NB110-60547]

Western Blot: STIM1 Antibody [NB110-60547] - Stromal interaction molecule 1 Antibody [NB110-60547] - Detection of STIM1 in HeLa whole cell extracts.
Immunocytochemistry/ Immunofluorescence: STIM1 Antibody - BSA Free [NB110-60547]

Immunocytochemistry/ Immunofluorescence: STIM1 Antibody - BSA Free [NB110-60547]

Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NB110-60547] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody.

Applications for STIM1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Knockdown Validated

1:1000

Western Blot

1:1000
Application Notes
This STIM1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In Western Blot a band is seen at ~77 kDa, representing the STIM1 protein. Two other non-specific bands of lower MW can also be seen.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STIM1

Stromal interaction molecule 1 (STIM1 or GOK) is a type 1 transmembrane protein that mediates store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. STIM1 is Ca2+ sensor that conveys information about ER lumen's Ca2+ load to store-operated Ca2+ channels. It exists as a single transmembrane protein located in ER membrane with a luminal EF hand and a cytosolic domain, which activates Ca2+ release-activated Ca2+ channels. Upon Ca2+ depletion, STIM1 translocates from ER to plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1 resulting in a strong Ca2+ influx. STIM1 is one of several genes located in imprinted gene domain of 11p15.5, an important tumor-suppressor gene region and alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. STIM1 implicates in early hematopoiesis by mediating attachment to stromal cells and defective STIM1 have been linked to immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Gene Symbol

STIM1

Additional STIM1 Products

Product Documents for STIM1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STIM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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