STIM1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44168
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for STIM1 Antibody
Immunogen
STIM1 Antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the N-terminus of human STIM1. (Uniprot: Q13586)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for STIM1 Antibody
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168]
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168] - Staining of human thyroid tissueImmunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168]
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168] - Staining of human testis tissue.STIM1 Antibody
Immunohistochemistry of Sheep STIM1 Antibody. Tissue: Thyroid. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase sheep secondary antibody at 1:10,000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.Applications for STIM1 Antibody
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry
5ug/ml
Western Blot
1ug/ml
Application Notes
This product is tested for ELISA and immunohistochemistry and suitable for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77kDa corresponding to the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (BSA)
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: STIM1
Additional STIM1 Products
Product Documents for STIM1 Antibody
Product Specific Notices for STIM1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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