STING/TMEM173 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24683

Novus Biologicals, part of Bio-Techne
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NBP2-24683SS
NBP2-24683
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Canine, Primate, Rhesus Macaque

Cited:

Human, Mouse, Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Block/Neutralize, ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all STING/TMEM173 Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide made to an internal portion of human STING/TMEM173 (between amino acids 310-360) [UniProt Q86WV6]

Reactivity Notes

Opossum, Zebrafish (83%), Xenopus (72%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STING/TMEM173 Antibody - BSA Free

Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Western Blot: STING/TMEM173 Antibody [NBP2-24683] - STING/TMEM173 expression was induced in human breast MDA-MB-231 cells followed by Western blotting using STING/TMEM173 Antibody antibody (1:1000). Only one specific band at an apparent molecular mass of 37 kDa was observed. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - RH-30 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with STING/TMEM173 Antibody at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha Tubulin Antibody (DM1A) (NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Total protein from THP-1, HT-29, U2OS cells and human spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml STING/TMEM173 Antibody in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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Applications for STING/TMEM173 Antibody - BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 34905508)

Flow Cytometry

1-5 ug/million cells

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

1:100 - 1:300

Immunohistochemistry-Frozen

reported in scientific literature (PMID 33745949)

Immunohistochemistry-Paraffin

1:100 - 1:300

Immunoprecipitation

Validated for Immunoprecipitation from YCharOS Inc. (ycharos.com)

Knockout Validated

Validated for Knockout from YCharOS Inc. (ycharos.com)

Western Blot

1 - 2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP2-24683 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STING/TMEM173

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-kB leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Long Name

Stimulator of Interferon Genes Protein/Transmembrane protein 173

Alternate Names

ERIS, MITA, MPYS, NET23, TMEM173, anti-STING, human STING, mouse STING

Gene Symbol

STING1

UniProt

Q86WV6 (Human)

Additional STING/TMEM173 Products

Product Documents for STING/TMEM173 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STING/TMEM173 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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