I’m curious about the mouse reactivity of your STING/TMEM173/MPYS antibody, Catalog Number NBP2-24683.

Name: STING/TMEM173 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-24683
Rating: 5 (1 reviews)

For our product NBP2-24683, the mouse reactivity for this product was determined by homology. The immunogen sequence for this antibody shares 94% homology to the mouse protein.

STING/TMEM173 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24683

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Citations (38)

Product Datasheet / COA / SDS / Protocols & Manuals

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP2-24683AF350

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Alexa Fluor 405

NBP2-24683AF405

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Alexa Fluor 488

NBP2-24683AF488

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Alexa Fluor 532

NBP2-24683AF532

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Alexa Fluor 594

NBP2-24683AF594

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Alexa Fluor 647

NBP2-24683AF647

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Alexa Fluor 700

NBP2-24683AF700

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Alexa Fluor 750

NBP2-24683AF750

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Allophycocyanin

NBP2-24683APC

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Allophycocyanin/Cy7

NBP2-24683APCCY7

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Biotin

NBP2-24683B

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CoraFluor 1

NBP2-24683CL1

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DyLight 350

NBP2-24683UV

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DyLight 405

NBP2-24683V

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DyLight 488

NBP2-24683G

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DyLight 550

NBP2-24683R

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DyLight 594

NBP2-24683DL594

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DyLight 650

NBP2-24683C

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DyLight 680

NBP2-24683FR

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DyLight 755

NBP2-24683IR

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FITC

NBP2-24683F

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HRP

NBP2-24683H

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Janelia Fluor 525

NBP2-24683JF525

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Janelia Fluor 549

NBP2-24683JF549

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Janelia Fluor 585

NBP2-24683JF585

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Janelia Fluor 635

NBP2-24683JF635

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Janelia Fluor 646

NBP2-24683JF646

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Janelia Fluor 669

NBP2-24683JF669

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mFluor Violet 450 SE

NBP2-24683MFV450

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mFluor Violet 500 SE

NBP2-24683MFV500

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mFluor Violet 610 SE

NBP2-24683MFV610

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NBP2-24683PE

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PE/Atto594

NBP2-24683PEATT594

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PE/Cy5.5

NBP2-24683PECY55

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PE/Cy7

NBP2-24683PECY7

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PerCP

NBP2-24683PCP

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Canine, Primate, Rhesus Macaque

Cited:

Human, Mouse, Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Block/Neutralize, ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all STING/TMEM173 Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide made to an internal portion of human STING/TMEM173 (between amino acids 310-360) [UniProt Q86WV6]

Reactivity Notes

Opossum, Zebrafish (83%), Xenopus (72%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STING/TMEM173 Antibody - BSA Free

Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Western Blot: STING/TMEM173 Antibody [NBP2-24683] - STING/TMEM173 expression was induced in human breast MDA-MB-231 cells followed by Western blotting using STING/TMEM173 Antibody antibody (1:1000). Only one specific band at an apparent molecular mass of 37 kDa was observed. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - RH-30 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with STING/TMEM173 Antibody at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha Tubulin Antibody (DM1A) (NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Total protein from THP-1, HT-29, U2OS cells and human spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml STING/TMEM173 Antibody in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Human colon cancer tissue section using STING/TMEM173 Antibody at 1:100 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated very weak cytoplasmic staining in columnar epithelial cells with a very strong signal in the secretory/goblet cells.

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Mouse lung tissue section using STING/TMEM173 Antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated mainly a cytoplasmic staining in the bronchiolar and alveolar epithelial cells.

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on U937 cells with NBP2-24683AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody NBP2-24683AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - HT-29 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with STING/TMEM173 Antibody at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on RH30 cells with STING/TMEM173 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Western Blot Shows Human STING/TMEM173 Specificity Using Knockout Cell Line.

Western blot shows lysates of THP-1 cell line and STING/TMEM173 knockout THP-1 cell line (KO). Nitrocellulose membrane was probed with STING/TMEM173 Antibody (Catalog # NBP2-24683) followed by an HRP-conjugated secondary antibody. A specific band was detected for STING/TMEM173 at approximately 41 kDa (as indicated) in the parental THP-1 cell line, but is not detectable in knockout THP-1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of STING/TMEM173 by Immunoprecipitation.

PMA-treated THP-1 lysates were prepared and immunoprecipitation was performed using 2.0 µg of STING/TMEM173 Antibody (Catalog # NBP2-24683) pre-coupled to Dynabeads protein A. Immunoprecipitated STING/TMEM173 was detected with STING/TMEM173 Antibody (Catalog # NBP3-18816). For western blot, NBP3-18816 was used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

STING/TMEM173 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

PMA-treated THP-1 WT and STING/TMEM173 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with STING/TMEM173 Antibody (Catalog # NBP2-24683) and with an Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

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Applications for STING/TMEM173 Antibody - BSA Free

Application

Recommended Usage

ELISA

reported in scientific literature (PMID 34905508)

Flow Cytometry

1-5 ug/million cells

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

1:100 - 1:300

Immunohistochemistry-Frozen

reported in scientific literature (PMID 33745949)

Immunohistochemistry-Paraffin

1:100 - 1:300

Immunoprecipitation

Validated for Immunoprecipitation from YCharOS Inc. (ycharos.com)

Knockout Validated

Validated for Knockout from YCharOS Inc. (ycharos.com)

Western Blot

1 - 2 ug/ml

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-24683 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STING/TMEM173

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-kB leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Long Name

Stimulator of Interferon Genes Protein/Transmembrane protein 173

Alternate Names

ERIS, MITA, MPYS, NET23, TMEM173, anti-STING, human STING, mouse STING

Gene Symbol

STING1

UniProt

Q86WV6 (Human)

Additional STING/TMEM173 Products

Product Documents for STING/TMEM173 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download IHC-P Protocol

Download Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for STING/TMEM173 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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STING/TMEM173 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24683

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all STING/TMEM173 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for STING/TMEM173 Antibody - BSA Free

Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Western Blot: STING/TMEM173 AntibodyBSA Free [NBP2-24683]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683]

Western Blot Shows Human STING/TMEM173 Specificity Using Knockout Cell Line.

Detection of STING/TMEM173 by Immunoprecipitation.

STING/TMEM173 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

Applications for STING/TMEM173 Antibody - BSA Free

ELISA

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Knockout Validated

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: STING/TMEM173

Long Name

Alternate Names

Gene Symbol

UniProt

Additional STING/TMEM173 Products

Product Documents for STING/TMEM173 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for STING/TMEM173 Antibody - BSA Free