Strep II Tag Antibody (517)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43735
![Western Blot: Strep II Tag Antibody (517) [NBP2-43735]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0004.jpg "Western Blot: Strep II Tag Antibody (517) [NBP2-43735]")
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 517
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Immunogen Amino acid sequence - ASWSHPQFEKGA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Strep II Tag Antibody (517)
![Western Blot: Strep II Tag Antibody (517) [NBP2-43735]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0006.jpg "Western Blot: Strep II Tag Antibody (517) [NBP2-43735]")
Western Blot: Strep II Tag Antibody (517) [NBP2-43735]
Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [517] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (517) [NBP2-43735]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-517-Immunocytochemistry-Immunofluorescence-NBP2-43735-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (517) [NBP2-43735]")
Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (517) [NBP2-43735]
Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (517) [NBP2-43735] - Analysis of Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep-tag antibody diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.![Western Blot: Strep II Tag Antibody (517) [NBP2-43735]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0003.jpg "Western Blot: Strep II Tag Antibody (517) [NBP2-43735]")
Western Blot: Strep II Tag Antibody (517) [NBP2-43735]
Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - Sample from human breast cell lysate (MDA-MB-231 cells). Absence of a band in the lane containing lysate from cells with empty vector confirm antibody is specific for Strep II Tag. Image submitted by a verified customer review.Applications for Strep II Tag Antibody (517)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using NBP2-43735 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol (pH7)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Strep II Tag
Additional Strep II Tag Products
Product Documents for Strep II Tag Antibody (517)
Product Specific Notices for Strep II Tag Antibody (517)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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