Strep II Tag Antibody (HL1252) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25699
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1252
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Strep II Tag Antibody (HL1252) - Azide and BSA Free
Immunogen
The immunogen used to generate this antibody corresponds to Strep II Tag.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Strep II Tag Antibody (HL1252) - Azide and BSA Free
Immunoprecipitation: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] -
Immunoprecipitation: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Immunoprecipitation of Strep epitope Tag protein from 293T whole cell extracts using 5 ug of Strep epitope Tag antibody [HL1252] (NBP3-25699). Western blot analysis was performed using Strep epitope Tag antibody [HL1252] (NBP3-25699). EasyBlot HRP-conjugated anti rabbit IgG antibody .
Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] -
Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Strep epitope Tag antibody [HL1252] detects Strep epitope Tag protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1252] (NBP3-25699) diluted at 1:500. Blue: Fluoroshield with DAPI .
Western Blot: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] -
Western Blot: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [HL1252] (NBP3-25699) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Strep II Tag Antibody (HL1252) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Strep II Tag
Additional Strep II Tag Products
Product Documents for Strep II Tag Antibody (HL1252) - Azide and BSA Free
Product Specific Notices for Strep II Tag Antibody (HL1252) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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