Streptavidin Antibody (S8C12cc)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-10022
Key Product Details
Species Reactivity
Non-species specific
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S8C12cc
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant streptavidin.
Specificity
No cross-reactivity with egg white avidin. Binds to streptavidin without interfering with the biotin-binding reaction.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Streptavidin Antibody (S8C12cc)
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Can be used to localize streptavidin, to amplify signals in immunocytochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Streptavidin
Alternate Names
SA V1; SA V2; Streptavidin V1; Streptavidin V2
Additional Streptavidin Products
Product Documents for Streptavidin Antibody (S8C12cc)
Product Specific Notices for Streptavidin Antibody (S8C12cc)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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