STRO-1 Antibody (STRO-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48356

Novus Biologicals, part of Bio-Techne
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NBP1-48356
NBP1-48356SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Lambda Clone # STRO-1

Concentration

1.0 mg/ml

View all STRO-1 Antibodies »

Product Specifications

Immunogen

Human CD34+ bone marrow cells.

Marker

Stromal Cell Surface Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Lambda

Scientific Data Images for STRO-1 Antibody (STRO-1)

Immunocytochemistry/ Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356]

Immunocytochemistry/ Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356]

Immunocytochemistry/Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356] - Antibody was tested at 1:50 in MG-63 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunocytochemistry: STRO-1 Antibody (STRO-1) [NBP1-48356]

Immunocytochemistry: STRO-1 Antibody (STRO-1) [NBP1-48356]

Immunocytochemistry: STRO-1 Antibody (STRO-1) [NBP1-48356] - Characterization of SCAPs: isolated SCAPs were positive for STRO-1 by immunocytochemistry; PBS served as a negative control. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2014/319651/), licensed under a CC-BY license.
Flow Cytometry: STRO-1 Antibody (STRO-1) [NBP1-48356]

Flow Cytometry: STRO-1 Antibody (STRO-1) [NBP1-48356]

Flow Cytometry: STRO-1 Antibody (STRO-1) [NBP1-48356] - STRO-1 antibody was tested at 1:400 in MG-63 cells using an Alexa Fluor 488 secondary (green) alongside unstained cells (red).
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Applications for STRO-1 Antibody (STRO-1)

Application
Recommended Usage

Flow Cytometry

1:400

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200
Application Notes
This STRO-1 Antibody is useful for Immunocytochemistry/Immunofluorescence and Flow Cytometry.

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STRO-1

As a part of experimental investigations focused on identifying reliable markers for mesenchymal stromal/stem cells (MSC), STRO-1 antibody was generated by using one of the several hybridomas that were generated by immunizing mice intrasplenically with human CD34+ bone marrow cells and despite lack of much details on exact identity of its cognate antigen, STRO-1 antibody has been heavily relied upon for the recognition/isolation of various types of MSCs for over past two decades. After initial screening against T-/B-cell lines, hybridomas followed further selection for reactivity against subpopulations of CD34-expressing cells as well as for the identification of STRO-1 clone, and additional tests identified STRO-1 antibody as of the IgM isotype which reacts with stromal cells in the adherent layer of long-term bone marrow cultures. STRO-1 has been considered the best-known MSC marker and its antibody has been employed primarily for FLOW application as well as for staining of putative MSCs. However, with the use of IHC and Western blot applications, recent studies have identified STRO-1 as a 75kd endothelial antigen in the vasculature of liver, lung and kidney tissues wherein it co-localized with endothelial marker vWF and also detected it in certain cancer cell lines namely PC3, DU145, MCF7, and K562.

Long Name

A Stromal Cell Precursor Surface Antigen

Alternate Names

STRO1

Additional STRO-1 Products

Product Documents for STRO-1 Antibody (STRO-1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STRO-1 Antibody (STRO-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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