SUG1 Antibody (25D5)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-345

Novus Biologicals, part of Bio-Techne
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NB100-345
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Chemotaxis, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 25D5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SUG1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encoding full-length SUG1 purified from E. coli.

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SUG1 Antibody (25D5)

Western Blot: SUG1 Antibody (25D5) [NB100-345]

Western Blot: SUG1 Antibody (25D5) [NB100-345]

Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000
Knockdown Validated: SUG1 Antibody (25D5) [NB100-345]

Knockdown Validated: SUG1 Antibody (25D5) [NB100-345]

Knockdown Validated: SUG1 Antibody (25D5) [NB100-345] - Representative immunoblot for SUG1 antibody knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695522/) licensed under a CC-BY license.
SUG1 Antibody (25D5)

Knockdown Validated: SUG1 Antibody (25D5) [NB100-345] -

Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. Representative immunoblots for PSMC5 and PSMB3 knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Additional knockdown data are presented in Supplementary Figure S6. (E) Knockdown of DSS1 as measured by mRNA level, as described in ‘Materials and Methods’ section. Error bar denotes ± SEM, n = 3.

Applications for SUG1 Antibody (25D5)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
IP reactivity reported in (PMID: 19660582). SUG1 antibody validated for CHIP from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4 using NB100-345 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUG1

Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.

Alternate Names

MSUG1 protein, p45, p45/SUG26S protease regulatory subunit 8, proteasome (prosome, macropain) 26S subunit, ATPase, 5, proteasome 26S ATPase subunit 5, Proteasome 26S subunit ATPase 5, Proteasome subunit p45, S8, SUG-1, SUG126S proteasome AAA-ATPase subunit RPT6, Tat-binding protein homolog 10, TBP10, thyroid receptor interactor 1, TRIP1Thyroid hormone receptor-interacting protein 1

Entrez Gene IDs

5705 (Human)

Gene Symbol

PSMC5

UniProt

P62195 (Human)

Additional SUG1 Products

Product Documents for SUG1 Antibody (25D5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUG1 Antibody (25D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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