SUG1 Antibody (25D5)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-345
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Knockdown Validated, Western Blot
Cited:
Chemotaxis, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 25D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encoding full-length SUG1 purified from E. coli.
Localization
Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SUG1 Antibody (25D5)
![Western Blot: SUG1 Antibody (25D5) [NB100-345]](https://resources.bio-techne.com/images/products/SUG1-Antibody-25D5-Western-Blot-NB100-345-img0002.jpg "Western Blot: SUG1 Antibody (25D5) [NB100-345]")
Western Blot: SUG1 Antibody (25D5) [NB100-345]
Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000![Knockdown Validated: SUG1 Antibody (25D5) [NB100-345]](https://resources.bio-techne.com/images/products/SUG1-Antibody-25D5-Knockdown-Validated-NB100-345-img0003.jpg "Knockdown Validated: SUG1 Antibody (25D5) [NB100-345]")
Knockdown Validated: SUG1 Antibody (25D5) [NB100-345]
Knockdown Validated: SUG1 Antibody (25D5) [NB100-345] - Representative immunoblot for SUG1 antibody knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695522/) licensed under a CC-BY license.
Knockdown Validated: SUG1 Antibody (25D5) [NB100-345] -
Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. Representative immunoblots for PSMC5 and PSMB3 knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Additional knockdown data are presented in Supplementary Figure S6. (E) Knockdown of DSS1 as measured by mRNA level, as described in ‘Materials and Methods’ section. Error bar denotes ± SEM, n = 3.Applications for SUG1 Antibody (25D5)
Application
Recommended Usage
Western Blot
1:1000
Application Notes
IP reactivity reported in (PMID: 19660582). SUG1 antibody validated for CHIP from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using NB100-345 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SUG1
Alternate Names
MSUG1 protein, p45, p45/SUG26S protease regulatory subunit 8, proteasome (prosome, macropain) 26S subunit, ATPase, 5, proteasome 26S ATPase subunit 5, Proteasome 26S subunit ATPase 5, Proteasome subunit p45, S8, SUG-1, SUG126S proteasome AAA-ATPase subunit RPT6, Tat-binding protein homolog 10, TBP10, thyroid receptor interactor 1, TRIP1Thyroid hormone receptor-interacting protein 1
Entrez Gene IDs
5705 (Human)
Gene Symbol
PSMC5
UniProt
Additional SUG1 Products
Product Documents for SUG1 Antibody (25D5)
Product Specific Notices for SUG1 Antibody (25D5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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