Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00025870-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Sulfatase Modifying Factor 2/SUMF2 Antibodies »

Product Specifications

Immunogen

SUMF2 (NP_056226, 26 a.a. ~ 125 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QATSMVQLQGGRFLMGTNSPDSRDGEGPVREATVKPFAIDIFPVTNKDFRDFVREKKYRTEAEMFGWSFVFEDFVSDELRNKATQPMKSVLWWLPVEKAF

Reactivity Notes

Human. Other species not tested.

Specificity

SUMF2 - sulfatase modifying factor 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3)

Western Blot: Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3) [H00025870-M02]

Western Blot: Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3) [H00025870-M02]

Western Blot: Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3) [H00025870-M02] - SUMF2 monoclonal antibody (M02), clone 4B3 Analysis of SUMF2 expression in A-431.
Sandwich ELISA: Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3) [H00025870-M02] - Detection limit for recombinant GST tagged SUMF2 is approximately 3ng/ml as a capture antibody.

Applications for Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Sulfatase Modifying Factor 2/SUMF2

The catalytic sites of sulfatases are only active if they contain a unique amino acid, C-alpha-formylglycine (FGly). The FGly residue is posttranslationally generated from a cysteine by enzymes with FGly-generating activity. The gene described in this record is a member of the sulfatase-modifying factor family and encodes a protein with a DUF323 domain that localizes to the lumen of the endoplasmic reticulum. This protein has low levels of FGly-generating activity but can heterodimerize with another family member - a protein with high levels of FGly-generating activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

FGE2

Entrez Gene IDs

25870 (Human)

Gene Symbol

SUMF2

OMIM

607940 (Human)

UniProt

NP_056226 (Human)

Additional Sulfatase Modifying Factor 2/SUMF2 Products

Product Documents for Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Sulfatase Modifying Factor 2/SUMF2 Antibody (4B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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