SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78069

Novus Biologicals, part of Bio-Techne
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NBP1-78069
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SUMO Activating Enzyme E1 (SAE1/UBA2) Antibodies »

Product Summary for SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

Immunogen

This purified SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 (SAE1) (SAE1) protein. (Uniprot: Q9UBE0)

Reactivity Notes

A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Modification

p Ser185

Specificity

This purified antibody is directed against human SUMO Activating Enzyme E1 protein. This antibody is specific for human SAE1 protein phosphorylated at S185. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This purified antibody is directed against human SUMO Activating Enzyme E1 (SAE1) protein. The product was purified from monospecific antiserum by affinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

Western Blot: SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody [NBP1-78069]

Western Blot: SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody [NBP1-78069]

Western Blot: SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody [NBP1-78069] - SUMO Activating Enzyme E1 (SAE1) antibody contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent de-phosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 2 ug/mL at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.

Applications for SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

Application
Recommended Usage

ELISA

1:5000-1:25000

Western Blot

2 ug/ml
Application Notes
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to phosphorylated SAE1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific antibody reacts with human SAE1 pS185 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.

Long Name

SUMO1 Activating Enzyme E1

Alternate Names

AOS1, SAE1, SUA1, UBA2, UBLE1A

Gene Symbol

SAE1

UniProt

Q9UBE0 (Human)

Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products

Product Documents for SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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