SUMO Activating Enzyme E1 (SAE1) Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44220
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This purified SUMO Activating Enzyme E1 (SAE1) Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SUMO Activating Enzyme E1 (SAE1) (SAE1) protein. (Uniprot: Q9UBE0)
Reactivity Notes
A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This purified antibody is directed against human SUMO Activating Enzyme E1 (SAE1) protein. The product was purified from monospecific antiserum by affinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody
![Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-UBA2-Antibody-Western-Blot-NBP2-44220-img0001.jpg "Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220]")
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220]
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220] - Left lane (-): 20 ug human HeLa whole cell protein. Right lane (+): 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with SUMO Activating Enzyme E1 (SAE1) Antibody (2 ug/mL) at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.Applications for SUMO Activating Enzyme E1 (SAE1) Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Western Blot
2ug/ml
Application Notes
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to SAE1 protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: SUMO Activating Enzyme E1 (SAE1/UBA2)
Long Name
SUMO1 Activating Enzyme E1
Alternate Names
AOS1, SAE1, SUA1, UBA2, UBLE1A
Gene Symbol
SAE1
UniProt
Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products
Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody
Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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