SUMO-interacting Motif (SIM) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30373

Novus Biologicals, part of Bio-Techne
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NBP3-30373
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SUMO-interacting Motif (SIM) Antibodies »

Product Specifications

Immunogen

between 25 and 75

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUMO-interacting Motif (SIM) Antibody

SUMO-interacting Motif (SIM) Antibody

Immunoprecipitation:SUMO-interacting Motif (SIM) AntibodyNBP3-30373] -

Immunoprecipitation:SUMO-interacting Motif (SIM) AntibodyNBP3-30373] -Detection of human SIMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SIMC1 antibody (NBP3-30373 lot 1) used for IP at 6 µg per reaction. SIMC1 was also immunoprecipitated by rabbit anti-SIMC1 antibody BL23153. For blotting immunoprecipitated SIMC1, NBP3-30373 was used at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SUMO-interacting Motif (SIM) Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SUMO-interacting Motif (SIM)

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that are enzymatically attached to target proteins by a process termed SUMOylation. This post-translational modification regulates many cellular processes including DNA transcription and repair, cell cycle progression, protein intracellular trafficking, and nuclear receptor activities. SUMO binding and/or interaction with proteins is mediated by short amino acid consensus sequences termed SUMO-interacting motifs (SIMs). To date, all SIMs appear to contain a hydrophobic core sequence that is either preceded or succeeded by an acidic region composed of either glutamate or aspartate residues or phosphorylated serine or threonine residues. The hydrophobic core of SIMs has been shown to interact with the alpha-helix and beta2-strand surfaces on SUMO proteins while the negatively charged residues surrounding the hydrophobic core appear to influence binding affinities and dictate binding preferences for the various SUMO isoforms. SIMs have been identified in numerous types of proteins including SUMO ligases (E3), transcription factors, and transcriptional repressors.

Alternate Names

SUMOinteracting Motif

Gene Symbol

SIMC1

Additional SUMO-interacting Motif (SIM) Products

Product Documents for SUMO-interacting Motif (SIM) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SUMO-interacting Motif (SIM) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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