SUMO2 Antibody (SPM621) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48455

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM621

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SUMO2 Antibodies »

Product Summary for SUMO2 Antibody (SPM621) - IHC-Prediluted

Immunogen

Recombinant human full-length SUMO2 protein. (Uniprot: P55854)

Reactivity Notes

Predicted to show a broad species reactivity.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for SUMO2 Antibody (SPM621) - IHC-Prediluted

Immunohistochemistry-Paraffin: SUMO2 Antibody (SPM621) - IHC-Prediluted [NBP2-48455]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SPM621) - IHC-Prediluted [NBP2-48455]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SPM621) - IHC-Prediluted [NBP2-48455] - Formalin-fixed, paraffin-embedded human Tonsil stained with SUMO-2 Mouse Monoclonal Antibody (SPM261).

Applications for SUMO2 Antibody (SPM621) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SUMO2

Function: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4; Subcellular location: Nucleus

Long Name

Small Ubiquitin-like Modifier 2

Alternate Names

Sentrin-2, SMT3B, SMT3H2

Gene Symbol

SUMO2

Additional SUMO2 Products

Product Documents for SUMO2 Antibody (SPM621) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO2 Antibody (SPM621) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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