SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47710

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SUMO2/1199

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all SUMO2 Antibodies »

Product Summary for SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free

Immunogen

Recombinant human SUMO2 protein (Uniprot: P55854)

Reactivity Notes

Predicted to show a broad species reactivity.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44477).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free

Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710] - Human Testicular Carcinoma stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710] - Human Tonsil stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710]

Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710] - Rat Heart stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
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Applications for SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SUMO2

Function: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4; Subcellular location: Nucleus

Long Name

Small Ubiquitin-like Modifier 2

Alternate Names

Sentrin-2, SMT3B, SMT3H2

Gene Symbol

SUMO2

Additional SUMO2 Products

Product Documents for SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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